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Identification of genes encoding plant vacuolar malate-transport proteins by functional expression in heterologous systems

ReferenceG01761
Principal Investigator / Supervisor Professor Andrew Smith
Co-Investigators /
Co-Supervisors
Institution University of Oxford
DepartmentPlant Sciences
Funding typeResearch
Value (£) 175,682
StatusCompleted
TypeResearch Grant
Start date 01/01/1995
End date 15/12/1998
Duration48 months

Abstract

The aim of this work is to identify the genes encoding vacuolar malate-transport proteins from plant cells by functional expression of the gene products in heterologous systems. The primary approach will involve complementation of a malate-transport mutant of Schizosaccharomyces pombe using a cDNA library from leaves of the CAM plant Kalanchoe daigremontiana. A secondary approach will involve characterization of mRNA fractions associated with expression of malate-transport activity in Xenopus oocytes. Identification of these genes will improve our understanding of the molecular mechanisms and control of solute accumulation and remobilization in plant vacuoles.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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