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Isolation of a gene from Arabidopsis thaliana conferring resistance to a phytotoxin

Reference	G01325
Principal Investigator / Supervisor	Professor John Turner
Co-Investigators / Co-Supervisors
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	225,133
Status	Completed
Type	Research Grant
Start date	27/06/1994
End date	27/06/1997
Duration	36 months

Abstract

We have isolated mutants of Arabidopsis thaliana resistant to the phytotoxin coronatine. These mutants, COI11, are also insensitive to the phytohormone, methyl jasmonate, and, remarkably, are male sterile. The pleiotropic phenotype in these mutants is regulated by a single locus, coi1. We propose to map the coi1 locus using PCR-based markers and RFLP markers. We shall physically map the gene to a genomic YAC or cosmid and confirm that COI1 has been cloned by T-DNA transformation of COI1 mutants to restore the wild-type phenotype. The cloned COI1 locus will provide a tool for the dissection of the signal transduction cascade that is involved in the action of methyl jasmonate and coronatine.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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