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Glycosylation of recombinant proteins expressed in transgenic animals
Reference
G01095
Principal Investigator / Supervisor
Professor Nigel Jenkins
Co-Investigators /
Co-Supervisors
Institution
University of Kent
Department
University of Kent Department
Funding type
Research
Value (£)
184,933
Status
Completed
Type
Research Grant
Start date
01/12/1993
End date
31/05/1997
Duration
42 months
Abstract
Recombinant human glycoproteins are produced in animal cell cultures and in transgenic animals, but our understanding of the glycosylation process and its control is far from complete. This programme will address key issues concerning the glycosylation process in the mammary gland, and how it is influenced by transgenic expression. It will also highlight the optimal physiological states in which to produce authentic human glycoproteins in transgenic animals. The carbohydrate structures of a model glycoprotein (alpha1-acid glycoprotein, AAT) expressed in the milk of transgenic mice will be analysed and compared to AAT from other sources, to define the mammary gland glycosylation profile. Key enzymes of the mammary gland N-glycosylation pathway will be measured, and their responses to environmental factors (e.g. diet, age, expression level) assessed.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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