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Making the most of a genome sequence: the application of global transcriptome and proteome analysis to Streptomyces coelicolor A3(2)

ReferenceFGT11407
Principal Investigator / Supervisor Professor Colin Smith
Co-Investigators /
Co-Supervisors		 Professor Stephen Oliver
Institution The University of Manchester
DepartmentLife Sciences
Funding typeResearch
Value (£) 460,964
StatusCompleted
TypeResearch Grant
Start date 01/04/2000
End date 01/04/2003
Duration36 months

Abstract

(i) Using PCR primers designed from the emerging Streptomyces coelicolor A3(2) genome sequence, whole-genome gene arrays will be produced, made available to the community, and their use (in terms of linearity and specificity) validated for the analysis of the transcriptome; (ii) a proteome map of the organism will be produced and validated; (iii) a suite of bioinformatic tools will be developed and made available for extracting biologically meaningful data from the proteome and transcriptome, which will be linked to the annotated genome database being produced at the Sanger Centre; and (iv) the transcriptome and proteome of a variety of mutant strains will be analysed and of the wild type during the transition to methylenomycin production in batch culture and during sporulation. (Joint with grants FGT11406 and FGT11408).

Summary

unavailable
Committee Closed Committee - Engineering & Biological Systems (EBS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Functional Genomics Toolkit (FGT) [1998]
Funding SchemeX – not Funded via a specific Funding Scheme
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