Award details

Complementation of essential yeast genes with human cDNAs: Assignment of function to human genes

Reference	FGT11380
Principal Investigator / Supervisor	Professor Stephen Oliver
Co-Investigators / Co-Supervisors
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	113,631
Status	Completed
Type	Research Grant
Start date	01/12/1999
End date	01/12/2001
Duration	24 months

Abstract

Large numbers of human genes are being sequenced and identified. Assignment of function is lagging far behind. We propose to use the model organism Saccharomyces cerevisiae to attack this problem by constructing a panel of yeast strains, in each of which, an essential gene is placed under the control of a down-regulatable promoter. The panel will be transformed with human cDNA libraries retrofitted into a yeast expression vector. We will down-regulate expression of the endogenous yeast targets and look for rescue (or partial rescue) by complementation. Examples of anti-complementation will also be assayed. The procedures will allow the categorisation of the function of human genes. (Joint with grant FGT11381).

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Functional Genomics Toolkit (FGT) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

FGT11381 Complementation of essential yeast genes with human cDNAs: Assignment of function to human genes

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