Award details

Inducible inhibition of gene expression using external guide sequence libraries and RNAseP

Reference	FGT11379
Principal Investigator / Supervisor	Dr Laurence Tiley
Co-Investigators / Co-Supervisors
Institution	University of Cambridge
Department	Veterinary Medicine
Funding type	Research
Value (£)	163,089
Status	Completed
Type	Research Grant
Start date	01/01/2000
End date	01/01/2003
Duration	36 months

Abstract

This proposal describes a technology for the conditional inhibition of prokaryotic or eukaryotic genes. It can be applied both specifically for studying the function of individual genes, and randomly for the identification of interesting genes based on phenotypic screening. Inhibition is achieved using short RNA molecules known as external guide sequences (EGS) that hybridise the target gene and redirect Ribonuclease P to cleave it. Because the inhibition is dependent on the expression of the EGS, the system can be made inducible, and can therefore target essential genes. The unusually small size of EGS allows the generation of libraries of EGS that represent all possible targets. EGS libraries can potentially be used to identify unknown genes associated with a particular phenotype. Sequencing a group of EGS that produce a particular phenotype should identify the gene(s) involved.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Functional Genomics Toolkit (FGT) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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