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Development of novel efficient vectors for activation tagging
Reference
FGT11352
Principal Investigator / Supervisor
Professor Chris Lamb
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
Biological Chemistry
Funding type
Research
Value (£)
111,280
Status
Completed
Type
Research Grant
Start date
01/03/2000
End date
01/03/2002
Duration
24 months
Abstract
We will develop and evaluate vectors for the large-scale functional characterisation of plant genes by activation tagging in Arabidopsis. A panel of related vectors are proposed for a) rapid generation of derivative loss-of- function alleles from activation-tagged alleles, b) conditionally enhanced expression of tagged alleles and c) suppression of closely related members of gene families. We will test the rationale underlying each vector in reconstruction experiments in planta and evaluate rapid mapping of T-DNA insertion sites in tagged lines by sequencing of TAIL-PCR products. The utility of these vectors in functional genomics will be evaluated by characterisation of several thousand activation-tagged lines for each vector.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Functional Genomics Toolkit (FGT) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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