Award details

Eukaryotic virus display as a technology for functional genomics

Reference	FGT11342
Principal Investigator / Supervisor	Professor Ian Jones
Co-Investigators / Co-Supervisors
Institution	University of Reading
Department	Animal and Microbial Sciences
Funding type	Research
Value (£)	161,186
Status	Completed
Type	Research Grant
Start date	01/02/2000
End date	31/01/2003
Duration	36 months

Abstract

This project aims to develop a system for functional genomics using the display of proteins on the surface of the eukaryotic baculovirus Autographa californica nuclear polyhedrosis virus, AcNPV. A basic requirement of the system, the directed expression of foreign proteins on the virus surface has already been established, but various methods for library creation and the selection of individual proteins by virtue of their phenotype now need to be investigated. The proposal suggests novel methods for generating multiple baculovirus recombinants making cDNA display libraries possible. It also describes methods for the recovery of live virus encoding desired functions by direct ligand interaction. Finally proposals for the display of two polypeptide chains are given opening the way for the development of a combinatorial display system.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Functional Genomics Toolkit (FGT) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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