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Microarrays-to-soluble proteins: exploitation of expression profiling through high-throughput protein production at the BCSB

Reference	EGH16115
Principal Investigator / Supervisor	Dr Paul Driscoll
Co-Investigators / Co-Supervisors	Dr Mark McAlister, Professor Laurence Pearl, Dr Renos Savva
Institution	University College London
Department	Structural Molecular Biology
Funding type	Research
Value (£)	1,337,456
Status	Completed
Type	Research Grant
Start date	29/04/2003
End date	28/07/2008
Duration	63 months

Abstract

Downstream of genome sequencing projects, the imperative of molecular biologists is the description of gene function. An important component of this activity is the determination of structure-activity relationships for proteins and enzymes, upon which the production of practically tractable macromolecules ultimately depends. We will build and operate a semi-automated protein production pipeline that builds upon the experience of the pilot project scheme of the Bloomsbury Centre for Structural Biology, and introduces a novel high-throughput procedure for the selection of proteins with maximum solubility, thereby biasing the chances of success in X-ray and NMR applications. The pipeline will be fed by the output of a number of DNA microarray projects in collaborating laboratories that are targeted to biomedically important areas, as well as in projects that are being developed in the BCSB. The outputs of the pipeline also have more general utility for the development of protein-chip based technologies.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	Structural Biology, Technology and Methods Development
Research Priority	X – Research Priority information not available
Research Initiative	Exploiting Genomics: Healthcare, Pharmaceuticals (EGH) [2001]
Funding Scheme	X – not Funded via a specific Funding Scheme

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