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Enzyomics - rapid multiplex determination of enzyme activity and selectivity
Reference
E20426
Principal Investigator / Supervisor
Professor Duncan Graham
Co-Investigators /
Co-Supervisors
Dr Barry Moore
Institution
University of Strathclyde
Department
Pure and Applied Chemistry
Funding type
Research
Value (£)
201,928
Status
Completed
Type
Research Grant
Start date
01/04/2004
End date
31/03/2006
Duration
24 months
Abstract
A new approach to determining enzyme activity and selectivity with minimal separations will be investigated. The basis of the approach lies in using the ultra sensitive analytical technique of surface enhanced resonance Raman scattering (SERRS) combined with modified enzyme substrates. The substrates are modified in such a way that they are masked and do not produce SERRS signals until enzymatic cleavage of a scissile bond occurs to release a specific SERRS label. The intensity of the signal relates to the enzyme activity and use of multiple labels and substrates allows determination of the selectivity of the enzyme. The sensitivity of the technique is such that a few hundred enzyme molecules can be assayed and various formats will be investigated to widen the applicability of the approach and optimise the sampling format.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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