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Characterisation of a novel N-linked general glycosylation pathway as a tool for glycoengineering
Reference
E20372
Principal Investigator / Supervisor
Professor Brendan Wren
Co-Investigators /
Co-Supervisors
Institution
London Sch of Hygiene and Trop Medicine
Department
Infectious and Tropical Diseases
Funding type
Research
Value (£)
269,132
Status
Completed
Type
Research Grant
Start date
19/04/2004
End date
18/08/2007
Duration
40 months
Abstract
Glycosylation plays a central role in the modification of proteins, from viruses to mammalian cells. But the lack of appropriate modification systems in bacteria has meant it is impossible to produce novel glycoproteins for study in simple hosts such as E. coli, until now. We have identified a novel N-linked general glycosylation pathway (Cjpgl) in Campylobacter jejuni and transferred it into E. coli, to produce recombinant PEB3 that appears structurally identical to the native glycoprotein (Wacker et al Science 2002 p1790-3). We propose to characterise the Cjpgl pathway and optimise the N-linked glycosylation process in E.coli, providing the basis for a unique tool for glycoengineering, with broad research and industrial applications.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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