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Laboratory evolution of alkene monooxygenase for improved chiral synthesis
Reference
E20252
Principal Investigator / Supervisor
Professor Thomas Smith
Co-Investigators /
Co-Supervisors
Institution
Sheffield Hallam University
Department
Faculty of Health and Wellbeing
Funding type
Research
Value (£)
209,294
Status
Completed
Type
Research Grant
Start date
01/12/2003
End date
28/02/2007
Duration
39 months
Abstract
Alkene monooxygenase (AMO) of Rhodococcus rhodochrous B-276 catalyses the NAD(P)H-dependent stereoselective oxidation of alkenes to produce high-value chiral epoxides such as epoxypropane. AMO requires the costly cofactor NADH or NADPH but this requirement by using hydrogen peroxide as the electron acceptor in place of oxygen, although both turnover and stereoselectivity are diminished. In order to produce an economic NAD(P)H independent biocatalyst, laboratory evolution methodology will be used to enhance the stereoselectivity and turnover of AMO when activated by hydrogen peroxide.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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