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De novo design of novel emulsifiers based on a protein scaffold for use in polymeric encapsulation of macromolecules
Reference
E19380
Principal Investigator / Supervisor
Dr Christopher van der Walle
Co-Investigators /
Co-Supervisors
Institution
University of Strathclyde
Department
Inst of Pharmacy and Biomedical Sci
Funding type
Research
Value (£)
131,868
Status
Completed
Type
Research Grant
Start date
01/02/2004
End date
30/04/2006
Duration
27 months
Abstract
A major obstacle for encapsulation of therapeutic proteins in polylactide-co-glycolide (PLGA) microspheres remains the emulsion phase which causes protein denaturation and loss of activity. The current use of additives and synthetic emulsifiers is not ideal and, surprisingly, protein emulsifiers have been largely ignored. This speculative proposal intends to explore their potential by building on the current understanding of characteristics typical of protein emulsifiers. Structure-prediction will be used to superpose these characteristics onto a human protein scaffold with minimal mutation. Mutants will be expressed and tested, allowing quantification of the resultant emulsifying activity with respect to the structural-parameters used.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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