BBSRC Portfolio Analyser
Award details
Vitrification of mammalian tissues
Reference
E19328
Principal Investigator / Supervisor
Professor David Pegg
Co-Investigators /
Co-Supervisors
Dr Martin Robinson
,
Dr Monica Wusteman
Institution
University of York
Department
Biology
Funding type
Research
Value (£)
236,144
Status
Completed
Type
Research Grant
Start date
25/08/2003
End date
24/08/2006
Duration
36 months
Abstract
The relatively low concentrations of cryoprotectant that are used in cell preservation prevent intracellular freezing but allow extracellular ice to form. This severely damaged tissues. Based on physical measurements and on experiments with cells, this study will investigate the ice-free preservation (vitrification) of elastic arteries and corneas. Sufficiently high concentrations of propane-1,2 diol and trehalose are used to prevent ice formation. The project aims to minimise the damage caused by exposure to these agents, to prevent devitrification (freezing during rewarming) by uniform and rapid electromagnetic heating and to avoid vitreous fractures by storage just below the glass transition temperature.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search