Award details

Green flourescent protein engineered to give novel chimaeric chemically functionalised intramolecular FRET mutants

Reference	E19152
Principal Investigator / Supervisor	Professor Kenneth Thomas Douglas
Co-Investigators / Co-Supervisors
Institution	The University of Manchester
Department	Manchester Pharmacy School
Funding type	Research
Value (£)	195,808
Status	Completed
Type	Research Grant
Start date	01/11/2003
End date	31/10/2006
Duration	36 months

Abstract

Green Fluorescent Protein (GFP) mutants with altered excitation and emission properties provide major tools for cell, developmental and molecular biology. This project combines UK/Japan expertise to rationally design and genetically engineer novel GFP mutants with surface Cys site(s) for site-specific chemical modification with a second fluorophore (e.g. Ca- switched) designed to act as FRET partner for GFP native fluorophore. Enzyme-susceptible sites will be introduced in the sequence between the GFP fluor and the engineered Cys, so that in the cell once the cognate enzyme has acted the FRET emission will be lost, and the emission will be switch to that of the GFP fluorophore, allowing ratio sensing for reporter gene applications.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search