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A new bio-analytical method for probing heme-thiolate proteins
Reference
E19149
Principal Investigator / Supervisor
Professor Stephen Chapman
Co-Investigators /
Co-Supervisors
Professor Lesley Yellowlees
Institution
University of Edinburgh
Department
Sch of Chemistry
Funding type
Research
Value (£)
181,800
Status
Completed
Type
Research Grant
Start date
29/09/2003
End date
28/09/2006
Duration
36 months
Abstract
A technique, for the use of pyridine derivatives as spectroscopic reporter molecules for heme thiolate proteins e.g. cytochromes P450 and nitric oxide synthases, will be developed. This will allow previously inaccessible thermodynamic parameters of these important biomolecules to be determined. The new approach will exploit the unusual ligation and spectral properties of P450- adducts of pyridine-derived molecules, exemplified by the preliminary study of the ligation of 4-cyanopyridine. The development of a simple and inexpensive spectrophotometric bio- assay from which the thermodynamic properties of heme-thiolate proteins, such as the heme reduction potential, can be accurately estimated, is our ultimate goal.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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