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Direct protein-engineering methods to generate specific microenvironments for in vivo cell studies
Reference
E19051
Principal Investigator / Supervisor
Professor Jeremy Lakey
Co-Investigators /
Co-Supervisors
Dr Mark Birch
,
Professor Andrew McCaskie
,
Professor Steve Yeaman
Institution
Newcastle University
Department
Inst for Cell and Molecular Biosciences
Funding type
Research
Value (£)
200,008
Status
Completed
Type
Research Grant
Start date
01/10/2003
End date
30/09/2006
Duration
36 months
Abstract
Self-assembled monolayers (SAM) are one method to create well- defined surfaces for cell growth where peptides and carbohydrates can be presented to guide desired development. So far, however, the use of large protein domains have been limited by the need to assemble proteins functionally and precisely at these interfaces. We have recently exploited natural membrane proteins as scaffolds for this form of assembly and in this project we will use new methods to allow recombinant proteins to be assembled directly in SAM. A core protein domain will be designed with different tails that allow for insertion into SAM and the domain will then be used as a scaffold onto which cell adhesion domains specific for particular cells will be added. Muscle and bone cells will be used and the proteins will be patterned using ion beam and photo- lithographic methods onto glass and polymer substrates.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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