Award details

Direct protein-engineering methods to generate specific microenvironments for in vivo cell studies

Reference	E19051
Principal Investigator / Supervisor	Professor Jeremy Lakey
Co-Investigators / Co-Supervisors	Dr Mark Birch, Professor Andrew McCaskie, Professor Steve Yeaman
Institution	Newcastle University
Department	Inst for Cell and Molecular Biosciences
Funding type	Research
Value (£)	200,008
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	30/09/2006
Duration	36 months

Abstract

Self-assembled monolayers (SAM) are one method to create well- defined surfaces for cell growth where peptides and carbohydrates can be presented to guide desired development. So far, however, the use of large protein domains have been limited by the need to assemble proteins functionally and precisely at these interfaces. We have recently exploited natural membrane proteins as scaffolds for this form of assembly and in this project we will use new methods to allow recombinant proteins to be assembled directly in SAM. A core protein domain will be designed with different tails that allow for insertion into SAM and the domain will then be used as a scaffold onto which cell adhesion domains specific for particular cells will be added. Muscle and bone cells will be used and the proteins will be patterned using ion beam and photo- lithographic methods onto glass and polymer substrates.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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