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Biological activities of atropic adenovirus in optic nerve regeneration

ReferenceE18035
Principal Investigator / Supervisor Dr Ana Maria Gonzalez
Co-Investigators /
Co-Supervisors
Professor Andrew Baird, Professor Martin Berry
Institution University of Birmingham
DepartmentWolfson Research Laboratories
Funding typeResearch
Value (£) 209,912
StatusCompleted
TypeResearch Grant
Start date 01/05/2004
End date 31/05/2007
Duration37 months

Abstract

The physical placement of cation-condensed plasmids at the site of optic nerve injury using gene activated matrices (GAM) obviates any need for ligand-mediated gene delivery. But although the effects of GAM are sustained (more than3 mo), efficiency is low. Adenoviruses (AD) in GAM however increase efficiency but the GAM specificity for regenerating axons and repair cells is lost. To address this problem, we propose to test atropic AD particles. Neutralised AD will be placed in GAM and gene expression monitored in repair cells and in the retinal ganglion cells (RGC) that retrogradely transport particles from the GAM. The effects of the anti-apoptotic gene bc12 and neuron specific promoters in GAM will be evaluated on RGC survival.

Summary

unavailable
Committee Closed Committee - Engineering & Biological Systems (EBS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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