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Hyperlum inescence of biomolecules on multiphoton excitation

Reference	E17993
Principal Investigator / Supervisor	Professor Roger Hugh Bisby
Co-Investigators / Co-Supervisors	Professor Stanley Botchway, Professor Anthony Parker
Institution	University of Salford
Department	Res Inst for the Built and Human Env
Funding type	Research
Value (£)	139,800
Status	Completed
Type	Research Grant
Start date	12/05/2003
End date	11/05/2005
Duration	24 months

Abstract

It is proposed to study the fluorescence (approximately 340 nm) and hyperluminescence (approximately 500 nm on fs laser multiphoton near-IR excitation) reported for indoles and catecholamines and suggested as probes for intracellular imaging of serotonin. Our preliminary experiments suggest that hyperluminescence arises from a transient photochemical product which will be identified by laser flash photolysis and resonance Raman experiments. Hyperluminescence arising from biomolecules will be characterised (spectra, lifetimes, fluorescence anisotropy) on multiphoton excitation with a Ti:sapphire laser at approximately 700-900 nm and evaluated as probes of protein rotation and flexibility and for imaging of cellular processes.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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