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SERRS beacons - quantitative multiplex analysis of gene sequence defects

Reference	E17890
Principal Investigator / Supervisor	Professor Duncan Graham
Co-Investigators / Co-Supervisors	Professor William Smith
Institution	University of Strathclyde
Department	Pure and Applied Chemistry
Funding type	Research
Value (£)	204,540
Status	Completed
Type	Research Grant
Start date	01/05/2003
End date	01/05/2006
Duration	36 months

Abstract

SERRS beacons are a new type of biomolecular sensor based on labelled oligonucleotides that utilise the spectroscopic techniques of fluorescence and surface enhanced resonance Raman scattering (SERRS). The SERRS beacons are designed to stick to metal surfaces which give highly efficient quenching of fluorescence and excellent SERRS. When the target molecule hybridises the quenching stops and fluorescence is observed. Quantitation will be based on the fluorescence signal which will be at levels lower than normal for a conventional beacon due to the more efficient quenching. The multiplexing is given by the SERRS signals and together with fluorescence will allow identification and quantitation of specific gene sequences at ultra low concentration levels. The format is ideal for development as a microarray system.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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