Award details

Overcoming systematic and intracellular barriers to synthetic gene delivery systems using novel polypropylenimine dendrimer derivatives

Reference	E17592
Principal Investigator / Supervisor	Professor Andreas Schätzlein
Co-Investigators / Co-Supervisors	Professor Ijeoma Uchegbu
Institution	University of Glasgow
Department	Medical Oncology
Funding type	Research
Value (£)	179,572
Status	Completed
Type	Research Grant
Start date	01/10/2002
End date	30/09/2006
Duration	48 months

Abstract

The aim of this proposal is to establish the influence of defined chemical and physicochemical modifications on the cellular transfer, processing and expression of plasmid DNA formulated in non-viral vector complexes utilising polypropylenimine dendrimer derivatives as tools. Systematic modification of carrier chemistry will be used to create DNA complexes with well-defined physicochemical properties, which will provide the tools to elucidate some of the factors that determine transfection efficiency on a cellular level. Using the polypropylenimine dendrimer series as models (and other carrier systems as controls), we will examine the importance of density of protonatable nitrogen, specific vs. non-specific uptake, and supramolecular structure and self-assembly for the ability of the systems to overcome biological barriers in vitro and in vivo.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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