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Mechanical loading modulates cytoskeletal organisation in living chondrocytes
Reference
E17114
Principal Investigator / Supervisor
Professor Martin Knight
Co-Investigators /
Co-Supervisors
Professor David Alan Lee
Institution
Queen Mary University of London
Department
IRC - Biomedical Materials
Funding type
Research
Value (£)
190,428
Status
Completed
Type
Research Grant
Start date
14/10/2002
End date
13/10/2006
Duration
48 months
Abstract
Mechanical loading is essential for the health of articular cartilage and may enhance tissue engineered repair. The cytoskeleton is involved in mechanotransduction although the influence of loading on the cytoskeleton is unclear. The tests hypothesis is that chondrocyte deformation induces cytoskeletal distortion and subsequent remodelling via a Ca2+:nitric oxide pathway with changes in cytoskeletal gene and protein expression. Isolated chondrocytes will be transfected with expression plasmids for GFP-tagged actin, tubulin and vimentin, seeded in agarose and visualised during and after compressive loading. A feedback system will be examined, whereby loading modulates cytoskeletal organisation and hence changes cellular mechanosensitivity.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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