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New tools for the directed evolution of enzyme function
Reference
E16590
Principal Investigator / Supervisor
Dr Cameron Neylon
Co-Investigators /
Co-Supervisors
Institution
University of Southampton
Department
Sch of Chemistry
Funding type
Research
Value (£)
194,668
Status
Completed
Type
Research Grant
Start date
22/04/2002
End date
21/04/2005
Duration
36 months
Abstract
Directed molecular evolution is an attractive approach to improving the activity of existing enzymes and in the longer term producing enzymes with new activities. I will develop a series of methods that will improve the efficiency of directed evolution experiments. These methods will allow the construction of improved libraries, identify optimal residues for randomisation, and allow high throughput sequence analysis of selected proteins. This will improve the efficiency of directed molecular evolution in producing modified enzymes and improve the prospects for producing novel biocatalysts. These methods will be applied to haloacid dehalogenases to produce enzymes with modified specificity for applications in biocatalysis.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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