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In vitro evaluation of tight junction-directed protein delivery vehicles

ReferenceE16523
Principal Investigator / Supervisor Dr Christopher van der Walle
Co-Investigators /
Co-Supervisors
Institution University of Bath
DepartmentPharmacy and Pharmacology
Funding typeResearch
Value (£) 112,396
StatusCompleted
TypeResearch Grant
Start date 01/05/2002
End date 30/11/2003
Duration19 months

Abstract

Protein delivery vehicles intended to specifically target and disrupt the intestinal tight junctions are to be investigated, initially used red-fluorescent microspheres conjugated at their surface with truncated and wild-type zona-occludens toxin (ZOT) and the putative binding domain of ZOT (G291RLCVQDG298). Localisation of these microsphere conjugates to the tight junctions of CaCo2 and IEC6 intestinal cell monolayers will be investigated by immunomicroscopy, co-staining the tight junctions with green fluorescence. Cell monolayers will then be used in transport studies to investigate the translocation properties of the microsphere conjugates in the apical to basolateral direction and their subsequent fate in order to gain a proof of concept.

Summary

unavailable
Committee Closed Committee - Engineering & Biological Systems (EBS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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