Award details

In vitro evaluation of tight junction-directed protein delivery vehicles

Reference	E16523
Principal Investigator / Supervisor	Dr Christopher van der Walle
Co-Investigators / Co-Supervisors
Institution	University of Bath
Department	Pharmacy and Pharmacology
Funding type	Research
Value (£)	112,396
Status	Completed
Type	Research Grant
Start date	01/05/2002
End date	30/11/2003
Duration	19 months

Abstract

Protein delivery vehicles intended to specifically target and disrupt the intestinal tight junctions are to be investigated, initially used red-fluorescent microspheres conjugated at their surface with truncated and wild-type zona-occludens toxin (ZOT) and the putative binding domain of ZOT (G291RLCVQDG298). Localisation of these microsphere conjugates to the tight junctions of CaCo2 and IEC6 intestinal cell monolayers will be investigated by immunomicroscopy, co-staining the tight junctions with green fluorescence. Cell monolayers will then be used in transport studies to investigate the translocation properties of the microsphere conjugates in the apical to basolateral direction and their subsequent fate in order to gain a proof of concept.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/X509296/1 In vitro evaluation of tight junction-directed protein delivery vehicles

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