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Controlled lysis of yeast in the gut for the delivery of vaccines probiotics and therapeutic agents
Reference
E15847
Principal Investigator / Supervisor
Dr Lubomira Stateva
Co-Investigators /
Co-Supervisors
Professor Stephen Oliver
,
Dr Geoffrey Warhurst
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
368,384
Status
Completed
Type
Research Grant
Start date
08/07/2002
End date
08/07/2005
Duration
36 months
Abstract
We propose to develop yeast strains for in-vivo delivery of non-secretable proteins, such as vaccines. The strains (either S. cerevisiae or S. boulardii) will contain 2-3 genes that play central roles in cell wall biogenesis under the control of regulatable promoters so that lysis can be switched on at will. These strains will be an integral part of a regulatory system, in which there is lysis of yeasts in the gut of the patient, followed by release of the recombinant proteins. This process which should allow the delivery of proteins for human and/or animal vaccines will be tested in animal models. A heterologous protein of therapeutic significance, e.g. phenylalanine ammonia lyase (PAL) will be used to demonstrate proof of concept.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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