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Exploiting cytoplasmic expression systems for therapeutic neurotrophic factor delivery to dorsal root ganglion neurons

Reference	E15812
Principal Investigator / Supervisor	Professor Ann Logan
Co-Investigators / Co-Supervisors	Professor Martin Berry, Professor Leonard Seymour
Institution	University of Birmingham
Department	Medical Sciences - Medicine
Funding type	Research
Value (£)	185,620
Status	Completed
Type	Research Grant
Start date	01/09/2001
End date	01/09/2004
Duration	36 months

Abstract

We will use novel cytoplasmic expression systems to enhance therapeutic neutrophic factor (NF) expression in post-mitotic dorsal root ganglia neurons (DRGN) in vitro and in vivo. Accordingly, we will evaluate two separate strategies of introducing into the cytoplasm of DRGN, RNA encoding either (i) T7 polymerase, an enzyme that promotes gene transcription together with a plasmid encoding an NF gene that is under T7 promoter control; or (ii) self-replicating subgenomic fragments (replicons) of positive strand RNA viruses that also encode NF. We anticipate that both vector strategies will increase the efficiency and the duration of NF expression in DRGN, thereby enhancing the neuroprotective and axon regenerative effects.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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