Award details

Biotinylation of enveloped viral gene therapy vectors and development of a scaleable purification process for such vectors

Reference	E15365
Principal Investigator / Supervisor	Dr David Darling
Co-Investigators / Co-Supervisors	Professor Farzin Farzaneh
Institution	King's College London
Department	Department of Molecular Medicine
Funding type	Research
Value (£)	174,716
Status	Completed
Type	Research Grant
Start date	19/11/2001
End date	19/11/2004
Duration	36 months

Abstract

Enveloped viruses are being developed as gene delivery vehicles for gene therapy. Therefore, purification processes generating virus preparations conforming to regulatory requirements need to be developed. Rather than trying to generate custom methodologies for this purpose, we propose to introduce a biotin tag into the envelop of Moloney murine leukaemia virus (MoMuLV) vectors, which will allow purification based on widely available materials and known technologies. Using MoMuLV as a model system, we anticipate that this purification technology will be transferable to other enveloped viral vectors, including those based on herpes simplex virus and lentiviruses. The results generated may therefore have substantial importance for the commercial production of a wide variety of gene therapy vectors. (Joint with 15376).

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

E15376 Biotinylation of enveloped viral gene therapy vectors and development of a scaleable purification process

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