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Functional genomics of cytochromes P450 via substrate-trapping technology

Reference	E15100
Principal Investigator / Supervisor	Professor David Lamb
Co-Investigators / Co-Supervisors	Professor Steven Kelly
Institution	Aberystwyth University
Department	Inst of Biological, Environ & Rural Sci
Funding type	Research
Value (£)	255,228
Status	Completed
Type	Research Grant
Start date	01/08/2001
End date	31/08/2004
Duration	37 months

Abstract

Numerous cytochrome P450 (CYP) gene sequences have been uncovered by genome sequencing projects which confronts biologists with the task of identifying the function of the gene products. We shall exploit our newly developed substrate-trapping technology to determine the roles that the CYP superfamily play in Streptomyces coelicolor A3(2) by: (i) heterologous expression in Escherichia coli and purification of each CYP; (ii) determination of CYP production within S. coelicolor through Western blot analysis; (iii) identification of each CYP substrate through the unique technology of substrate-trapping. This approach will be generic for the functional genomic analysis of the cytochromes P450 superfamilies across all the Kingdoms of Life and through identification of their natural substrates, and will allow the efficient exploitation of such enzymes in biotechnology.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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