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Combinatorial optimisation in directed evolution experiments : a systematic approach

Reference	E15073
Principal Investigator / Supervisor	Dr Michael Winson
Co-Investigators / Co-Supervisors	Professor David Corne, Professor Douglas Kell, Dr Leighton Pritchard, Mr Jem Rowland
Institution	Aberystwyth University
Department	Inst of Biological, Environ & Rural Sci
Funding type	Research
Value (£)	265,904
Status	Completed
Type	Research Grant
Start date	01/05/2001
End date	30/09/2004
Duration	41 months

Abstract

The aims of this project are: 1) To develop a novel method for determining the average mutation rates in directed evolution (DE) experiments based on sloppy PCR; 2) Thereby to calibrate and optimise the mutation rate in three DE systems; 3) To establish the optimal size of DNA fragments in directed recombination in these systems; and 4) Finally, therein systematically to determine the independence or otherwise of evolution at separate sites on a protein.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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