Award details

Novel methods for preparing enzyme-oligonucleotide conjugates

Reference	E15046
Principal Investigator / Supervisor	Professor Keith R Fox
Co-Investigators / Co-Supervisors	Professor Tom Brown
Institution	University of Southampton
Department	Centre for Biological Sciences
Funding type	Research
Value (£)	184,636
Status	Completed
Type	Research Grant
Start date	01/11/2001
End date	31/10/2004
Duration	36 months

Abstract

We propose to develop a novel and versatile strategy for reversibly tethering enzymes to oligonucleotides, which is based on the availability of His-tag proteins. For this purpose we will prepare a novel monomer for use in oligonucleotide synthesis which is based on nitrilo-triacetic acid (NTA). This will be attached to the 5 prime-end of oligonucleotides during automated synthesis. Enzyme conjugates can then be made by simply mixing these oligonucleotides with appropriate His-tag proteins forming strong coordination complexes in the presence of Ni2+. This strategy will be used to prepare novel sequence specific nucleases for use in DNA manipulation as well as oligonucleotide-enzyme conjugates for use in nucleic acid-based detection methods.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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