Award details

Using the Salmonella typhimurium FNR protein to target therapeutic gene expression in hypoxic sites in diseased tissues

Reference	E14966
Principal Investigator / Supervisor	Professor J Green
Co-Investigators / Co-Supervisors	Professor Claire Elizabeth Lewis
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	179,220
Status	Completed
Type	Research Grant
Start date	12/11/2001
End date	12/11/2004
Duration	36 months

Abstract

The delivery of drugs to their site of action is a fundamental barrier to the full exploitation of post-genomics in healthcare. For example, one of the most crucial aspects of gene therapy for cancer continues to be targeting of therapeutic gene expression to solid tumours which possess anoxic/hypoxic regions that are difficult to treat conventionally. Thus, we propose to investigate the utility of the anaerobic transcription factor FNR for targeting therapeutic gene expression to hypoxic sites of tumours using S. typhimurium as the delivery vehicle. We will: construct the necessary strains; use an FNR-regulated reporter/therapeutic gene to monitor gene expression in S. typhimurium during invasion of tumour spheroids; and modify promoter architecture and FNR properties to improve the targeting of therapeutic gene expression.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search