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Engineering a novel biocatalytic route for R(-) carvone production from S(-) alpha-pinene
Reference
E14586
Principal Investigator / Supervisor
Emeritus Professor David Jonathan Leak
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
240,088
Status
Completed
Type
Research Grant
Start date
26/06/2001
End date
25/10/2004
Duration
40 months
Abstract
The ultimate aim of this project is to create whole cell biocatalysts capable of converting pinene to carvone. A key component of this is the reprogramming of pinene oxide lyase to produce carveol. To do this we will be testing the hypothesis that, in the absence of detailed 3D structural information, enzyme catalysis can be reprogrammed by adopting a 3 step procedure (1) knocking out catalytic residues to create null mutants; (2) inserting equivalent residues at a number of alternative sites, determined by 'rational scanning mutagenesis', (3) catalyst refinement based on established principles of protein evolution. A 3 step pathway will then be assembled by co-expressing pinene monooxygenase, the modified lyase and carveol dehydrogenase.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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