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Targeted delivery of DNA into the cytoplasm of epithelial cells for the treatment of prostate cancer
Reference
E13913
Principal Investigator / Supervisor
Professor Leonard Seymour
Co-Investigators /
Co-Supervisors
Dr Thierry Bettinger
,
Dr Martin Read
Institution
University of Oxford
Department
Oncology
Funding type
Research
Value (£)
294,378
Status
Completed
Type
Research Grant
Start date
01/08/2000
End date
01/08/2003
Duration
36 months
Abstract
Poor intracellular processing limits the therapeutic usefulness of non-viral vectors for gene delivery. This study combines strategies for sophisticated trigger mechanisms designed to release free DNA within the cytoplasm with enhanced nuclear-targeting of DNA. Nanoparticles formed by condensation of DNA with thiol-bearing poly(ethyleneimine) will be stabilised by oxidation. DNA released by reduction of the disulphide linkages within cells will be targeted to the nucleus by using nuclear-localisation signal peptides, or DNA sequences known to bind nuclear-localising transcription factors. These strategies should provide improved transgene expression within cells in vitro and in vivo for application in the treatment of prostate cancer.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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