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Targeting long DNA sequences using DNaseI-PNA conjugates

Reference	E13750
Principal Investigator / Supervisor	Professor Bernard Connolly
Co-Investigators / Co-Supervisors
Institution	Newcastle University
Department	School of Cell and Molecular Biosciences
Funding type	Research
Value (£)	213,128
Status	Completed
Type	Research Grant
Start date	01/11/2000
End date	01/11/2003
Duration	36 months

Abstract

It is proposed to develop nucleases with specificity for extended DNA sequences using conjugates consisting of a DNA targeting agent (peptide-nucleic acid, PNA) and a nuclease (deoxyribonuclease I, DNaseI). PNA is able to bind specifically to double stranded DNA via triplex formation and strand invasion. Mutants of DNaseI have been described which retain catalytic activity but are deficient in DNA binding. Linking DNaseI variants, with poor intrinsic DNA binding ability, to PNA should result in reagents that hydrolyse DNA in response to sequences 10 or more base pairs in length. Such reagents are expected to be of tremendous utility in the manipulation of large genomes and also have therapeutic potential e.g. by specific degradation of viral or bacterial DNA in the presence of host cell DNA.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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