BBSRC Portfolio Analyser
Award details
A novel pathway for heterologous protein export in bacteria
Reference
E13320
Principal Investigator / Supervisor
Professor Colin Robinson
Co-Investigators /
Co-Supervisors
Institution
University of Warwick
Department
Biological Sciences
Funding type
Research
Value (£)
179,288
Status
Completed
Type
Research Grant
Start date
01/05/2000
End date
30/09/2003
Duration
41 months
Abstract
The aim is to develop entirely novel strategies for the export of heterologous proteins from Escherichia coli. The recently-discovered twin-arginine translocation (Tat) pathway offers unique possibilities due to its ability to export fully-folded proteins, and this project will involve a detailed examination of the commercial applicability of the pathway. The work will involve 1) determination of the size/types of heterologous proteins that can be exported; 2) assessment of the efficiency of disulphide bond formation in proteins targeted by this route; 3) optimisation of Tat- specific targeting signals; and 4) engineering of strains capable of increased translocation flux.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search