Award details

Formulation of lipid-enveloped gene vectors and vaccines

Reference	E13159
Principal Investigator / Supervisor	Professor Nigel Slater
Co-Investigators / Co-Supervisors	Professor Oya Alpar, Professor W Irwin
Institution	University of Cambridge
Department	Chemical Engineering and Biotechnology
Funding type	Research
Value (£)	175,736
Status	Completed
Type	Research Grant
Start date	18/09/2000
End date	07/04/2004
Duration	43 months

Abstract

We seek methods by which stable, freeze-dried vector preparations can be manufactured that are based upon a sound understanding of the physical and molecular phenomena involved in the lyophilisation process. The proposal addresses current issues in freeze-drying of lipid-enveloped gene vectors with regard to three key areas: 1) the relative stabilisation of reporter-gene bearing liposomes and lipid-enveloped viruses (HSV and MuLV) in terms of transfection efficiency and infectivity following freezing of the aqueous suspension; 2) the physico- chemical basis of vector stabilisation in terms of the molecular interactions between particle and excipients during freezing; and 3) the impact of freezing conditions upon lyophilisation.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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