Award details

Direct sensitive and quantitative detection of viruses using rupture force spectroscopy

Reference	E12967
Principal Investigator / Supervisor	Professor David Klenerman
Co-Investigators / Co-Supervisors	Professor Chris Abell, Professor Tony Minson
Institution	University of Cambridge
Department	Chemistry
Funding type	Research
Value (£)	116,229
Status	Completed
Type	Research Grant
Start date	20/11/1999
End date	20/11/2001
Duration	24 months

Abstract

We propose to develop a new low cost and sensitive way to detect viruses directly. It will be based on a new principle for biosensing called rupture force spectroscopy. A monoclonal antibody will be anchored to the surface of a quartz crystal microbalance and the virus is detected by the breakage of the bonds attaching the virus to the surface at a particular force. We have performed proof of concept experiments with phage and seek funds to extend this work to the medically important viruses of herpes and influenza. We will develop a protocol for making reproducible measurements on these viruses and demonstrate the quantification, sensitivity and selectivity of the method. We will then study the effect of sample contaminants on the measurement to simulate clinical samples.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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