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Combinatorial screening in micromachined silicon arrays: A route to novel diagnostic reagents

Reference	E12965
Principal Investigator / Supervisor	Professor Jonathan Cooper
Co-Investigators / Co-Supervisors	Professor Anthony Cass
Institution	University of Glasgow
Department	Institute of Cancer Studies
Funding type	Research
Value (£)	200,783
Status	Completed
Type	Research Grant
Start date	01/02/2000
End date	01/01/2003
Duration	36 months

Abstract

Previously, the development of biomolecular receptors for diagnostics has focused on using as-purified proteins. We now propose a novel method for developing new diagnostic reagents, using combinatorial methods to produce a wide variety of different binding sites, either by random mutagenesis and selection or rational design. Proteomic chip based arrays will then be used to measure the activity of the new receptors produced (each with a range of affinities, appropriate for different applications, including new drug discovery and analytical biotechnology). To screen the large number of proteins, we will develop a three dimensional proteomic microarray, based upon silicon micromachining. In contrast with previous planer technologies, this novel 'biochip' will have a number of significant advantages, including a high throughput, and a greater sensitivity, both as a consequence of the chip geometry.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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