Award details

Smarter assays : a `brighter luciferase'

Reference	E12914
Principal Investigator / Supervisor	Professor James Murray
Co-Investigators / Co-Supervisors	Professor Christopher Lowe, Dr Laurence Tisi
Institution	University of Cambridge
Department	Institute of Biotechnology
Funding type	Research
Value (£)	229,744
Status	Completed
Type	Research Grant
Start date	01/02/2000
End date	01/02/2003
Duration	36 months

Abstract

Firefly luciferase has important applications in diagnostics and in the monitoring of microbial contamination. However, assays require large amounts of reagents and consume the thermolabile enzyme non-catalytically. Preliminary experiments indicate that we can engineer luciferase to alter its thermostability, affinity for ATP and the kinetics of light emission. Here we propose to (i) lower the Km values of luciferase for ATP and its substrate, luciferin, (ii) increase its Kcat, (iii) reduce irreversible inactivation, leading to a more sensitive, economic and versatile luciferase for a wide range of diagnostic applications. (MOD joint grant scheme)

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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