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Award details
Synthesis and applications of porphocyanine-oligodeoxynucleotide conjugates as photoactive antisense agents
Reference
E12509
Principal Investigator / Supervisor
Professor Ross Boyle
Co-Investigators /
Co-Supervisors
Dr Julie Lloyd
,
Dr Minnie O'Farrell
Institution
University of Hull
Department
Chemistry
Funding type
Research
Value (£)
172,220
Status
Completed
Type
Research Grant
Start date
01/10/2000
End date
24/05/2004
Duration
43 months
Abstract
Conjugates of porphocyanine, a novel class of expanded porphyrin, and oligodeoxynucleotides will be synthesised and assayed for potential as photoactive antisense agents. Conjugates of porphocyanine and oligodeoxynucleotides will be investigated using a rapid screening system based on inhibition of a reporter gene which expresses beta- galactosidase. Promising conjugates will then be assayed for their ability to specifically inhibit human oncogene expression in cells expressing normal and mutant human ras p21. Fluorescence microscopy will be used to study uptake and intracellular distribution of conjugates; substituents on the porphocyanine will be modified to optimise these factors.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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