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Enhancing the degradative activity of bacteria by genetic augmentation with P450cam to provide novel catabolic pathways
Reference
E12155
Principal Investigator / Supervisor
Professor Luet Wong
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Oxford Chemistry
Funding type
Research
Value (£)
212,768
Status
Completed
Type
Research Grant
Start date
01/01/2000
End date
01/01/2003
Duration
36 months
Abstract
The catabolic range of the haem monooxygenase cytochrome P450cam has been extended to recalcitrant chlorinated aromatics. The main objectives of this study are (1) to refine and extend this activity to PCBs, and (2) to return this ability to selected Pseudomonas strains to genetically augment the degradative ability of co-inhabiting indigenous bacteria in a bioreactor. The activity of introduced pseudomonad(s) will be monitored using reporter genes, and the impact on, and potential transfer of novel genes to, indigenous bacteria assessed by total community genomic profiling. This novel approach will increase bioreactor capabilities, and generate organisms with acquired novel pathways that degrades contaminants not catabolised by wild types.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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