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A novel fluorescence technique for studying proteins in vivo
Reference
E11895
Principal Investigator / Supervisor
Professor Kevin Brindle
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Biochemistry
Funding type
Research
Value (£)
233,896
Status
Completed
Type
Research Grant
Start date
10/01/2000
End date
30/04/2003
Duration
40 months
Abstract
Tagging of cellular proteins with fluorescent probe molecules is an exciting and rapidly expanding technique in cell biology which promises new insights into cell function. A problem with existing techniques is that they invariably involve the introduction of a bulky chromophore, which may perturb the interaction of the protein with other molecules in the cell. We have developed a novel, minimally invasive, labelling method in which the label is, in effect, a single oxygen atom. The purpose of this study will be to further develop this methodology and to evaluate its performance in comparison with the widely used method involving labelling with green fluorescent protein (GFP). In developing the technique we will investigate the complexation and location of two proteins in the yeast Saccharomyces cerevisiae.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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