Award details

Development of prokaryotic ( blue green ) and eukaryotic freshwater algae as molecular biosensors

Reference	E11489
Principal Investigator / Supervisor	Professor Lesley Glover
Co-Investigators / Co-Supervisors	Professor Christopher Howe, Professor Andrew Porter
Institution	University of Aberdeen
Department	School of Medical Sciences
Funding type	Research
Value (£)	257,822
Status	Completed
Type	Research Grant
Start date	01/02/2000
End date	01/02/2004
Duration	48 months

Abstract

We will genetically modify a freshwater prokaryotic blue green alga (cyanobacterium) with bacterial lux genes, chromosomally and as a multicopy plasmid. We will also engineer a freshwater eukaryotic alga by chromosomal transformation with both fused luxAB constructs and the eukaryotic luc gene. The resulting bioluminescent algae will be used to: correlate bioluminescence with cellular activity: determine the response of lux/luc transformed algae as biosensors: compare the sensitivity and specificity of the two algal biosensors with each other and with our existing bacterial biosensors: determine the bioavailability of a variety of pollutants in liquid effluent: apply algal biosensors as indicators of acute and chronic toxic response.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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