Award details

High resolution whole-field three dimensional fluorescence lifetime imaging

Reference	E11253
Principal Investigator / Supervisor	Professor Paul Michael William French
Co-Investigators / Co-Supervisors	Professor M. John Lever, Professor Tony Wilson
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	271,572
Status	Completed
Type	Research Grant
Start date	01/02/1999
End date	01/11/2001
Duration	33 months

Abstract

The aim of this project is to investigate the use of structured excitation in fluorescence microscopy, pioneered at Oxford University, to provide whole-field 3-D sectioning with comparable resolution to confocal microscopy, combined with fluorescence lifetime imaging to map the local chemical and physical fluorophore environment. This novel imaging modality will be implemented using state-of-the-art diode-pumped ultrafast laser technology pioneered at Imperial College. Single and multi-photon excitation of fluorophores with ultrashort pulses in the visible and near infrared spectral region will be applied to standard phantoms and biological tissue in vitro. (Joint with grant E11178).

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	BioImaging (BI) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

E11178 High resolution whole-field three-dimensional fluorescence lifetime imaging

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