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The dynamics of biomolecules at interfaces studied by pulsed-force atomic force microscopy

ReferenceE11169
Principal Investigator / Supervisor Professor Clive Roberts
Co-Investigators /
Co-Supervisors
Professor Martyn Davies, Professor Saul Tendler
Institution University of Nottingham
DepartmentSch of Pharmacy
Funding typeResearch
Value (£) 177,508
StatusCompleted
TypeResearch Grant
Start date 08/02/1999
End date 08/08/2001
Duration30 months

Abstract

We propose to utilise and refine pulsed-force atomic force microscopy (AFM), a novel oscillation form of AFM, to study biomolecular distribution, interaction and functionality at interfaces. The significant potential of AFM using modified probes to quantify and spatially locate individual biomolecular interactions is apparent, however, to realise this potential a more robust and faster data acquisition rate is required. We will demonstrate the potential of pulsed-force to provide such progress (e.g. x100 increase in data rate) through mapping of protein adhesion (biocompatibility) to micropatterned self-assembled monolayers and polymers and also specific receptor-ligand interactions.

Summary

unavailable
Committee Closed Committee - Engineering & Biological Systems (EBS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative BioImaging (BI) [1998]
Funding SchemeX – not Funded via a specific Funding Scheme
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