Award details

Integrated miniaturised macromolecule separation and detection systems using fourier transform and correlation techniques

Reference	E11126
Principal Investigator / Supervisor	Professor Nick Goddard
Co-Investigators / Co-Supervisors	Professor Peter Fielden
Institution	The University of Manchester
Department	Chem Eng and Analytical Science
Funding type	Research
Value (£)	166,227
Status	Completed
Type	Research Grant
Start date	01/10/1999
End date	28/02/2003
Duration	41 months

Abstract

Current electrophoretic methods of separation of biological macromolecules are slow, require relatively large amounts of material and considerable skill to obtain reproducible results. The project will address these difficulties in four ways: (i) By miniaturisation of the separation channel in planar polymer substrates so the channel can be filled with a liquid polymer buffer solution instead of a gel; (ii) By using electroosomotic/electrophoretic sample injection into the separation channel to provide multiple reproducible injections and hence improved signal to noise; (iii) By using arrays of optical waveguide detectors along the channel to increase the signal to noise ratio; and (iv) By using Fourier Transform and correlation techniques to extract the electropherogram from the signal variations as the separation proceeds. These improvements will be applied to the separation of DNA for sequencing and genetic fingerprinting applications.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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