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Award details
Monitoring of mutagenic and toxic chemicals by fluorescence induction and detection. Phase 2.
Reference
E11056
Principal Investigator / Supervisor
Professor Richard Walmsley
Co-Investigators /
Co-Supervisors
Professor Peter Fielden
,
Professor Nick Goddard
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
200,152
Status
Completed
Type
Research Grant
Start date
01/01/1999
End date
01/01/2001
Duration
24 months
Abstract
In phase 1 we designed, constructed and tested a multianalyte, biological effect sensor using a one channel flow-through detector for real time measurement of both Saccharomyces cerevisiae cell concentration and GFP fluorescence. The DNA damage responsive promoter of the RAD54 gene drove GFP production. In phase 2 we propose: 1. Construction of a multiple channel, flow injection monitor to allow continuous calibration against blanks and standards, and multiple on-line sampling. 2. A yeast strain development programme to store and deliver a genetically stable yeast strain in the monitor, with fluorescent reporters from both genotoxic and general stress.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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