Award details

Identification and analysis of nucleic acid point mutations by surface enhanced resonance raman scattering SERRS

Reference	E11015
Principal Investigator / Supervisor	Professor Duncan Graham
Co-Investigators / Co-Supervisors	Professor William Smith
Institution	University of Strathclyde
Department	Pure and Applied Chemistry
Funding type	Research
Value (£)	161,480
Status	Completed
Type	Research Grant
Start date	01/12/1999
End date	01/04/2003
Duration	40 months

Abstract

A fast, reliable, sensitive methodology for detection of point mutations is proposed. It utilises the ultra high sensitivity, high selectivity, robustness and reliability of surface enhanced resonance Raman scattering. It requires a specific molecular handle and a suitable receptor and will accurately identify the base present at any location without amplification or separation stages, or will identify several consecutive bases which commonly cause uncertainty with existing protocols. Applications include detection of genes responsible for rare disease, analysis of the molecular details of identified genes, identification of specific mutations and rapid analysis of rates and sites of mutation. The novel concepts provide a completely new approach to biological analysis which could be applied more generally with huge advantages over existing procedures.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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