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A combined heat/oxygen flux metabolite probe to detect initiation and control suppression of apoptosis in cell culture
Reference
E10985
Principal Investigator / Supervisor
Dr Ross King
Co-Investigators /
Co-Supervisors
Institution
Aberystwyth University
Department
Inst of Biological, Environ & Rural Sci
Funding type
Research
Value (£)
195,282
Status
Completed
Type
Research Grant
Start date
13/01/1999
End date
01/10/2002
Duration
45 months
Abstract
An on-line probe for precise measurement of oxygen flux will be combined with heat flux as a powerful monitor of aerobic and anaerobic processes in animal cells growing in bioreactor culture. The value of the metabolic probes will be demonstrated by their detection of the decisive mitochondrial events in environmentally-initiated apoptosis in hybridomas. This will be contrasted with the protection afforded to cells by the overexpression of the bcl-2 protein in cells. Metabolic changes will be correlated with events well-documented by flow cytometry. The ability of exogenous agents to suppress chemically-induced apoptosis in continuous culture will be metabolically monitored to reveal clues to both mechanism and possible restorative strategies. The probes will reveal the metabolic consequences of overcoming bcl-2 protein protection with chemotherapeutic drugs.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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