Award details

Directed evolution of cytochrome p450 from Streptomyces griseus for enhanced biocatalysis and bioremediation

Reference	E10309
Principal Investigator / Supervisor	Professor Steven Kelly
Co-Investigators / Co-Supervisors	Professor Douglas Kell, Professor David Lamb
Institution	Aberystwyth University
Department	Inst of Biological, Environ & Rural Sci
Funding type	Research
Value (£)	228,642
Status	Completed
Type	Research Grant
Start date	01/02/1999
End date	01/12/2002
Duration	46 months

Abstract

Cytochrome P450 105 D1 (CYP105) from Streptomyces griseus is a soluble enzyme capable of metabolising an unprecedented array of diverse organic pollutants, as well as many drugs and other chemicals. We will utilise directed evolution coupled to microbiological/genetic selection procedures to obtain mutants with higher broad- substrate catalytic activity. Novel approaches to the detection of high-yielding strains will include the use of Fluorescence Activated Cell Sorting and DRASTIC non- invasive FTIR analyses. The mutants isolated will be subject to DNA sequence analysis and characterised enzymologically. This combination of strategies is best suited to the discovery of more efficient biocatalysts beyond those produced through natural evolution.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search